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Image Search Results
Journal: eLife
Article Title: Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment
doi: 10.7554/eLife.68571
Figure Lengend Snippet: ( A ) Male WT, Chil1 -/- , Cd44 -/- mice were treated with acetaminophen (APAP) (n = 4 mice/group). After 3 hr, mice were sacrificed and Mɸs were isolated to measure mRNA levels of various adhesion molecules, including selectin P ligand ( Selplg ), Cd40 , melanoma cell adhesion molecule ( Mcam ), Fc receptor ( Fcr ), intercellular adhesion molecule 1 ( Icam1 ), lymphocyte function-associated antigen 1 ( Lfa1 ), von Willebrand factor ( Vwf ), and podoplanin ( Pdpn ). ( B, C ) Wild-type (WT) mice were treated with APAP. Chil1 -/- and Cd44 -/- mice were treated with PBS or rmChi3l1 followed by APAP challenge simultaneously and mice were sacrificed 3 hr after APAP (n = 3 mice/group). ( B ) Mɸs were isolated and mRNA levels of Pdpn in Mɸs were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). ( C ) Immunofluorescence (IF) staining of liver sections for podoplanin and F4/80 is shown and the proportions of Mɸs that express Pdpn were quantified, Scale bar, 25 μm. ( D–F ) Chil1 -/- mice reconstituted with rmChi3l1 were treated with either Ctrl IgG or α-podoplanin Ab for 16 hr and subsequently challenged with APAP. ( D ) Serum levels of ALT and ( E ) liver histology were evaluated 24 hr after APAP treatment (n = 6 mice/group). Scale bar, 250 μm. ( F ) IF staining for intrahepatic platelets (CD41 + ) and Mɸs (F4/80+) was performed 3 hr after APAP (n = 3 mice/group). Scale bar, 25 μm. One-way ANOVA were performed in A–C . Two-tailed, unpaired Student’s t-test was performed in D .
Article Snippet: Antibody , Syrian hamster polyclonal IgG Ctrl IgG for
Techniques: Isolation, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Immunofluorescence, Staining, Two Tailed Test
Journal: eLife
Article Title: Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment
doi: 10.7554/eLife.68571
Figure Lengend Snippet: Mɸs were isolated from wild-type (WT) mice treated with acetaminophen (APAP) for 3 hr. The cells were treated in vitro with either control IgG (Ctrl IgG) or an anti-podoplanin antibody (α-podoplanin Ab) before incubation with platelets. Immunofluorescence (IF) staining was performed to detect podoplanin on Mɸs and C-type lectin-like receptor 2 (Clec-2) on platelets. Scale bar, 25 μm.
Article Snippet: Antibody , Syrian hamster polyclonal IgG Ctrl IgG for
Techniques: Isolation, In Vitro, Control, Incubation, Immunofluorescence, Staining
Journal: eLife
Article Title: Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment
doi: 10.7554/eLife.68571
Figure Lengend Snippet: Acetaminophen (APAP) overdose induces chitinase 3-like-1 (Chi3l1) expression, which binds CD44 on Mɸs and promotes Mɸs-mediated platelets recruitment through podoplanin/Clec-2 (C-type lectin-like receptor 2) interaction. Recruited platelets further contribute to APAP-induced liver injury (AILI).
Article Snippet: Antibody , Syrian hamster polyclonal IgG Ctrl IgG for
Techniques: Expressing
Journal: eLife
Article Title: Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment
doi: 10.7554/eLife.68571
Figure Lengend Snippet:
Article Snippet: Antibody , Syrian hamster polyclonal IgG Ctrl IgG for
Techniques: Recombinant, Diagnostic Assay, Intravital Microscopy